Bioinformatics Combined With Biological Experiments to Explore the Promotion of Lung Metastasis by CCL18 in the Immune Microenvironment of Colorectal Cancer - PubMed
3 hours ago
- #Bioinformatics
- #Lung Metastasis
- #CCL18
- Colorectal cancer (CRC) is a prevalent malignancy where lung metastasis is a common extra-abdominal spread with poor prognosis.
- This study aimed to identify key genes involved in CRC lung metastasis using bioinformatics and biological experiments.
- Analysis of gene expression data (GEO) identified differentially expressed genes (DEGs) between primary tumors and pulmonary metastatic CRC.
- CCL18 was pinpointed as a key gene through prognostic survival analysis, TCGA data, and single gene GSEA.
- Biological experiments, including macrophage co-culture and transwell assays, confirmed CCL18's role in promoting CRC cell invasion, metastasis, and proliferation.
- The findings suggest CCL18 as a potential target for improving prognosis in CRC patients with lung metastasis.