Single-cell multiomics gene regulatory landscape reveals impaired spermatogonial stem cells and macrophage-driven inflammaging during testicular aging - PubMed
3 hours ago
- #macrophages
- #spermatogonial stem cells
- #testicular aging
- Single-cell multiomics analysis reveals epigenetic drivers of testicular aging in mice.
- Spermatogonial stem cells (SSCs) are most vulnerable, with downregulation of transcription factor ZFX impairing SSC maintenance.
- Somatic niche disruption includes impaired blood-testis barrier in Sertoli cells and steroidogenic dysfunction in Leydig cells.
- Expansion of CD206+H2-Eb1- macrophages drives local inflammation via CXCL13, disrupting the SSC niche.
- Cross-species analysis identifies conserved aging-associated regulons like BRCA1 and E2F1.
- ZFX and CXCL13 are nominated as potential therapeutic targets for age-related reproductive decline.