Uncovering spatially resolved functional genomics with CRISPR screen sequencing - PubMed
3 hours ago
- #cancer immunology
- #CRISPR screen
- #spatial genomics
- Development of SPAC-seq, a high-throughput spatial CRISPR screen platform, and TARDIS, a statistical toolkit for analyzing spatial perturbation data.
- SPAC-seq and TARDIS link gene perturbations to spatial phenotypes and pathways, revealing immune suppression and macrophage polarization mechanisms in metastasis.
- Identified roles of specific genes: Icam1 loss promotes metastasis, and Cd44 in CD8+ T cells regulates spatial phenotypes via interactions with Spp1 on macrophages.
- Demonstrated a model linking transcription factors and chemokine receptors to couple cell states with chemotaxis.
- The platform provides a framework for studying spatially resolved functional genomics across various biological and disease contexts.