A genetic tool targeting brain GPR75 - PubMed
2 hours ago
- #GPR75
- #Obesity Research
- #Knockin Mouse
- Researchers generated a new GPR75-GFP-Ires-Cre knockin mouse strain with Cre expression driven by the endogenous GPR75 promoter.
- GFP is fused to the C-terminal of GPR75, and both Cre and GFP colocalize with endogenous GPR75 expression.
- The GPR75-GFP fusion protein remains functionally normal without altering susceptibility to diet-induced obesity (DIO).
- Using this mouse strain, GPR75 was found to be broadly expressed throughout the brain, mainly in the cytoplasm of neurons.
- This genetic tool enables study of the neural basis of GPR75 in mediating DIO, addressing previous unclear anatomical locations.