Experimental evolution reveals adaptive pathways to reduced antibiotic susceptibility in Pseudomonas aeruginosa biofilms - PubMed
2 hours ago
- #Pseudomonas aeruginosa
- #Antibiotic Resistance
- #Biofilm Evolution
- Experimental evolution was used to study how Pseudomonas aeruginosa biofilms develop reduced susceptibility to tobramycin and meropenem.
- Two biofilm models were employed: surface-attached biofilms and cystic fibrosis sputum-like aggregates.
- Whole-genome sequencing identified known and novel genetic adaptations linked to decreased antibiotic susceptibility.
- Mutations in oprD and irlR were associated with reduced susceptibility to meropenem.
- Adaptation to tobramycin involved mutations in fusA1, anr, ccoQ1, ptsP, phoQ, and nppA1A2BCD.
- The study highlights the diverse genetic strategies P. aeruginosa uses to resist antibiotics in biofilms.