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Myeloid ATF3 Protects Against Liver Fibrosis by Modulating the Extracellular Microenvironment and Macrophage Inflammatory Signaling - PubMed

3 hours ago
  • #liver fibrosis
  • #macrophage signaling
  • #ATF3
  • Activating transcription factor 3 (ATF3) is a stress-inducible transcription factor that regulates inflammatory responses, and its role in liver fibrosis is not fully understood.
  • Myeloid-specific ATF3 knockout mice (Atf3fl/fl LysM-Cre+) were used to study liver fibrosis induced by carbon tetrachloride (CCl4).
  • ATF3 deficiency worsened liver injury, shown by increased serum ALT/AST, collagen deposition, fibrosis scores, and hepatic stellate cell activation.
  • While total macrophage numbers were unchanged, ATF3 loss led to more Ly6C+ infiltrating macrophages and fewer Kupffer cells.
  • Mechanistically, ATF3-deficient livers had higher chemokine expression, reduced MMPs, increased TIMP1, and enhanced TGF-β1/SMAD signaling.
  • Myeloid ATF3 acts as a protective factor by limiting inflammation and extracellular matrix buildup in liver fibrosis.
  • Targeting ATF3 pathways could be a potential therapy for fibrotic liver disease.