Allele-specific CRISPR perturbation of the imprinted Dlk1-Dio3 domain reveals regulation of BMP-NOTCH-VEGF signaling in embryonic organogenesis - PubMed
3 hours ago
- #Embryonic Development
- #Genomic Imprinting
- #CRISPR-Cas9
- Researchers used allele-specific CRISPR perturbation to study the imprinted Dlk1-Dio3 domain's role in embryonic organogenesis.
- Four mouse models (HOMO, MK, PK, WT) were created to examine parent-of-origin-specific effects on development.
- ScRNA-seq at E14.5 showed that homozygous or maternally targeted inactivation leads to developmental arrest, disrupting BMP-NOTCH-VEGF signaling.
- Organ-specific pathologies include placental angiogenesis issues, impaired liver hematopoietic progenitor differentiation, and cardiac defects.
- The study identifies Dlk1-Dio3 as a critical hub integrating signaling pathways during organogenesis.