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Species-specific roles of SP1 in bovine and human embryonic genome activation and early embryonic development - PubMed

3 hours ago
  • #Preimplantation development
  • #Embryonic genome activation
  • #SP1 transcription factor
  • Embryonic genome activation (EGA) initiates the embryonic program and transitions to first lineage specification.
  • Single-cell RNA-seq on bovine embryos shows major EGA starts asynchronously at the 8-cell stage.
  • SP1, a transcription factor activated during minor EGA, has motifs enriched in accessible chromatin during major EGA in bovine and human embryos.
  • SP1 deficiency causes morula arrest in bovine embryos and impairs EGA in human embryos.
  • Multiomics analysis reveals SP1 promotes early lineage gene expression by modulating chromatin states in bovine and targets key EGA genes in human embryos.
  • The study highlights conserved and species-specific roles of SP1 in EGA and early mammalian development.