Overcoming redundancy in the Arabidopsis TREHALOSE-6-PHOSPHATE PHOSPHATASE family reveals connections to development and iron homeostasis - PubMed
4 hours ago
- #TPP genes
- #shoot development
- #iron homeostasis
- The study investigates the redundant functions of the 10 TREHALOSE-6-PHOSPHATE PHOSPHATASE (TPP) genes in Arabidopsis.
- Using expression profiling, CRISPR-Cas9 editing, and metabolite profiling, it shows that TPPI and TPPJ are expressed in shoot meristem boundaries, similar to maize RAMOSA3.
- Knockout of all 10 TPP genes leads to increased branching, earlier flowering, and chlorosis with low iron levels.
- Metabolite changes include higher trehalose 6-phosphate (Tre6P), lower trehalose, and altered sugar and iron-associated metabolites.
- Developmental and iron-responsive genes are upregulated in mutants, while photosynthesis-related genes are repressed.
- Findings suggest TPP genes redundantly regulate shoot architecture, sugar metabolism, iron homeostasis, and photosynthesis through Tre6P signaling.