Red-shifted GRAB acetylcholine sensors for multiplex imaging in vivo - PubMed
3 hours ago
- #in vivo neuroscience
- #neurotransmitter imaging
- #acetylcholine sensors
- New red fluorescent G-protein-coupled receptor activation-based acetylcholine sensors (rACh1h) enable detection of ACh release in various brain regions such as nucleus accumbens, amygdala, hippocampus, and cortex.
- These red-shifted GRAB sensors allow multiplex imaging, permitting simultaneous recording of ACh along with other neuromodulators using techniques like fiber photometry, mesoscopic imaging, and two-photon imaging.
- The development supports high spatiotemporal resolution in vivo, aiding studies on complex behaviors regulated by interactions between ACh and other neuromodulators.