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Single-Cell and Bulk Transcriptomics Uncover the Cellular Ecosystem of Vascular Invasion in Intrahepatic Cholangiocarcinoma - PubMed

4 hours ago
  • #tumor microenvironment
  • #vascular invasion
  • #intrahepatic cholangiocarcinoma
  • Single-cell and bulk transcriptomics identified a distinct subset of cancer-associated fibroblasts (tCAFs) enriched in vascular invasion (VI)-positive intrahepatic cholangiocarcinoma (ICC).
  • tCAFs are associated with hypoxia and angiogenesis pathways, promoting VI primarily through VEGF signaling interactions with endothelial cells.
  • Key functional genes in tCAFs include SLC2A1, PTGS2, and PLOD2; SLC2A1 inhibition suppressed endothelial sprouting and tumor progression in models.
  • The study unveils a hypoxia-tCAFs-endothelial cells axis driving VI in ICC and identifies SLC2A1 as a potential therapeutic target.