A Single-Cell Transcriptomic Atlas of the Ovine Rumen Microbiome Characterizes Lineage-Specific Metabolic Shifts Associated with Host Heat Tolerance - PubMed
3 hours ago
- #rumen microbiome
- #single-cell transcriptomics
- #heat tolerance
- Development of a 25-minute enzymatic lysis strategy using smRandom-seq for single-cell transcriptomic profiling of the sheep rumen microbiome.
- Profiling of 60,748 cells across 21 samples captured 213 genera and 662 species, including a 0.303% recovery of methanogenic archaea.
- Unsupervised clustering identified seven cross-species functional clusters, revealing spatial coupling between microbial lifestyle and metabolic specialization.
- Analysis of host thermal adaptation shows host resilience linked to rapid transcriptional activation of key energy-metabolism clusters.
- Lineage-specific metabolic shift toward a glycolytic phenotype in Anaerovibrio lipolyticus contributes to a compensatory 'nutritional sparing' effect associated with host resilience.