Ready-to-load MHC-I nanoparticles for high-throughput T cell screening studies - PubMed
3 hours ago
- #T cell screening
- #Artificial antigen presentation
- #MHC-I nanoparticles
- Researchers developed VLP-Open HLA, a multivalent, loadable virus-like particle platform that can display up to 60 peptide/HLA molecules.
- The platform enables staining of antigen-specific CD8+ T cells and serves as a screening tool for orphan T cell receptors (TCRs).
- When combined with costimulation, VLP-Open HLA acts as an artificial antigen-presenting platform to induce antigen-specific T cell activation and expansion.
- It offers a simple alternative to using autologous antigen-presenting cells in experimental immunology.
- The platform is adaptable to include multiple HLA allotypes and costimulatory molecules as mosaic nanoparticles.