Targeted Delivery of mRNA to the Heart via Extracellular Vesicles or Lipid Nanoparticles - PubMed
4 hours ago
- #cardiac therapeutics
- #extracellular vesicles
- #mRNA delivery
- Cardiac progenitor cell-derived extracellular vesicles (CPC-EVs) show enhanced cardiac selectivity for mRNA delivery compared to non-cardiac EVs and lipid nanoparticles (LNPs).
- Systemic administration of CPC-EVs results in reduced liver accumulation and a localized, limited cytokine response, unlike LNPs which trigger a widespread pro-inflammatory reaction.
- Intramyocardial injection of CPC-EVs leads to efficient mRNA uptake and robust VEGF-A protein expression with minimal transcriptomic perturbation in cardiac tissue.
- VEGF-A mRNA delivery via CPC-EVs enhances angiogenic potential, as evidenced by increased CD31 and α-SMA expression and vessel formation in ex vivo aortic ring assays.
- CPC-EVs are a promising platform for targeted cardiac mRNA delivery, offering reduced off-target effects and improved safety profiles compared to traditional methods.