Ensilication preserves high-molecular weight native DNA for clinical long-read sequencing - PubMed
3 hours ago
- #DNA preservation
- #long-read sequencing
- #ambient storage
- Ensilication encapsulates DNA within silica matrices to preserve it at ambient temperature for up to 30 days.
- This method yields sequencing performance equivalent to conventional freezing at -80°C, with no significant differences in read length, variant-calling accuracy, or genome-wide CpG methylation.
- Ensilicated DNA maintains fragment integrity under accelerated weathering and tolerates repeated handling better than frozen samples.
- The technique enables diagnostic-quality native long-read sequencing without cold-chain infrastructure, supporting ambient storage and transport.
- In clinical applications, ensilicated DNA has resolved de novo variants in rare genetic disorders and detected consistent methylation patterns.