ELOF1 is a core component of the promoter-proximal paused RNA polymerase II complex - PubMed
4 hours ago
- #ELOF1
- #RNA polymerase II pausing
- #transcription regulation
- ELOF1 is identified as a required factor for promoter-proximal pausing of RNA polymerase II in metazoans, acting with DSIF and NELF.
- In cells, ELOF1 degradation reduces pause duration, and in assays, it enhances pausing by DSIF and NELF under physiological conditions.
- Cryo-EM shows ELOF1, along with DSIF and NELF, sterically clashes with TFIIF, counteracting its positive effects on transcription.
- The study establishes ELOF1 as a core component of the paused RNA polymerase II complex, completing the cellular pausing mechanism.