Development of a robust method to derive human trophoblast stem cells from late-gestation placentas and its application to preeclampsia - PubMed
4 hours ago
- #placenta research
- #preeclampsia
- #trophoblast stem cells
- A robust method has been developed to derive human trophoblast stem cells (hTSCs) from late-gestation placentas, overcoming previous limitations.
- The technique involves transient expression of a p53 dominant negative mutant, SALL4, and shRNAs against cyclin-dependent kinase inhibitors.
- hTSCs were successfully derived from placentas of patients with early-onset preeclampsia (PE), providing a model to study this pregnancy complication.
- PE-derived hTSCs show impaired trophoblast invasion and reduced secretion of placental growth factor, aligning with known trophoblast abnormalities in PE.
- This method offers a technological basis for investigating pregnancy complications linked to trophoblast dysfunction, addressing a scarcity of experimental models.