Facile inactivation of a Streptomyces global regulator using a versatile base editing plasmid for secondary metabolism perturbation across multiple species - PubMed
5 hours ago
- #Base editing
- #Global regulator
- #Secondary metabolism
- Developed a versatile base editing plasmid to inactivate the global regulator wblA by introducing a stop codon.
- The single guide RNA (sgRNA) was designed to be applicable across multiple Streptomyces species due to high sequence conservation of wblA homologs.
- Inactivation of wblA in Streptomyces lavendulae FRI,5 led to increased production of lavencidins and indigoidine, with gene complementation restoring metabolite levels.
- In Streptomyces virginiae NBRC12827, wblA inactivation induced extensive secondary metabolism perturbation through transcriptional changes in biosynthetic gene clusters.
- The method facilitates the discovery of new secondary metabolites by awakening cryptic biosynthetic gene clusters in diverse Streptomyces species.