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Helical reconstruction of amyloids in cryoSPARC - PubMed

2 hours ago
  • #amyloids
  • #helical-reconstruction
  • #cryoSPARC
  • Amyloid filament structures are linked to over 50 diseases, but some have beneficial roles.
  • CryoSPARC is underutilized for amyloid helical reconstruction compared to RELION, needing guidelines.
  • Guidelines for helical reconstruction of amyloids in cryoSPARC are presented using public datasets.
  • Amyloid-specific parameter tuning in cryoSPARC is crucial to overcome current limitations.
  • Unsupervised workflows in cryoSPARC could aid high-throughput studies and drug development.