Distinct and overlapping roles of MutLγ, Mus81-Mms4, and STR in meiotic Holliday junction processing - PubMed
4 hours ago
- #Meiotic crossover resolution
- #MutLγ nuclease
- #Double Holliday junction processing
- MutLγ is uniquely capable of imposing crossover-specific resolution on ZMM-bound double Holliday junctions.
- Mus81-Mms4 and the STR complex can access crossover-designated intermediates but generate mixed or exclusively noncrossover products.
- Top3-Rmi1 has an Sgs1-independent role in maintaining ZMM-dHJ architecture and preventing conversion into MutLγ-refractory species.
- ZMM proteins establish a hierarchy, rather than absolute selectivity, in dHJ processing to favour MutLγ-directed crossovers while preserving alternative routes.
- An engineered yeast system stabilizes pre-existing ZMM-bound dHJs and enables conditional pathway-specific resolution without cell-cycle advance.