Ophiopogon japonicus alleviates pulmonary fibrosis through modulation of HIF1A/TUBB3 axis-associated M2 macrophage polarization and arginine metabolic alterations - PubMed
4 hours ago
- #Pulmonary Fibrosis
- #Macrophage Polarization
- #Traditional Chinese Medicine
- Ophiopogon japonicus aqueous decoction alleviates bleomycin-induced pulmonary fibrosis in mice, reducing weight loss, lung dysfunction, and tissue damage.
- Sixteen potential bioactive constituents were identified, with Ophiopogonin D suggested as a candidate active compound.
- The anti-fibrotic effect is linked to modulation of the HIF1A/TUBB3 axis, reducing M2 macrophage polarization and correcting arginine metabolic dysregulation.
- Multi-omics and bioinformatics analyses, including network pharmacology and machine learning, identified HIF1A as a hub target and TUBB3 as a key feature gene.
- Structural simulations indicate Ophiopogonin D can stably bind to HIF-1α and TUBB3 proteins, supporting its potential role in the mechanism.