Uncovering the electrical synapse proteome in retinal neurons via in vivo proximity labeling - PubMed
3 hours ago
- #proteomics
- #retinal neurons
- #electrical synapse
- The study aimed to uncover the proteome of electrical synapses in retinal neurons using in vivo proximity labeling techniques.
- Two strategies were employed: using a Cx36-EGFP mouse strain with a GFP-nanobody-TurboID fusion in AII amacrine cells, and generating a transgenic zebrafish line expressing Cx35.1-TurboID.
- Over 50 new proteins were identified, including scaffold proteins, adhesion molecules, and cytoskeleton regulators, alongside known interactors like ZO-1 and ZO-2.
- Sipa1l3 was highlighted as a new scaffold protein at electrical synapses, interacting with ZO-1, ZO-2, and Cx36, suggesting a key functional role.
- The findings provide the first detailed view of the electrical synapse proteome, showing high evolutionary conservation between species and likely applicability to other neural contexts.