Poising and connectivity of emergent human developmental enhancers in the transition from naive to primed pluripotency - PubMed
3 hours ago
- #pluripotency transition
- #poised enhancers
- #3D genomics
- Poised enhancers (PEs) are common in primed human pluripotent stem cells (hPSCs) but scarce in naive hPSCs.
- PEs form abundant chromosomal contacts with developmental genes, with poising emerging early in the transition from naive to primed pluripotency, while contacts show diverse dynamics.
- Degradation of Polycomb repressive complex 2 (PRC2) early in the transition weakens PE connectivity, unlike inhibition of its H3K27 methyltransferase activity.
- PE contacts persist after developmental activation or CRISPRa targeting and can mediate long-range gene induction.