H3K9me3 controls epidermis morphogenesis by regulating RNA Pol II dynamics at developmental promoters and enhancers - PubMed
a day ago
- #epigenetics
- #RNA polymerase II
- #epidermis development
- H3K9me3 deposition is dynamic during epidermis morphogenesis and essential for lineage diversification in mice.
- Ablation of H3K9me3 methyltransferases (Suv39h1, Suv39h2, Setdb1) leads to failure in keratinocyte differentiation, skin barrier formation, hair follicle development, and Merkel cell specification.
- Single-cell transcriptomics shows aberrant cell fates with mixed identities and dysregulated lineage-specific and non-lineage transcription programs.
- H3K9me3 primarily restricts RNA Pol II transcription initiation at developmental promoters and enhancers, with minimal effect on promoter-proximal pause release.
- Suv39h1, Suv39h2, and Setdb1 cooperatively control gene expression, establishing H3K9me3 as a critical determinant in skin organogenesis.