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Systemic metabolic, hormonal, and glycomic remodeling during a 72-hour fast in healthy adults: a pilot study - PubMed

3 hours ago
  • #prolonged fasting
  • #glycomic adaptation
  • #metabolic remodeling
  • A 72-hour water-only fast in healthy adults induces a distinct biphasic response in metabolic and hormonal markers, with significant changes in total cholesterol, C-reactive protein, thyroid-stimulating hormone, and free triiodothyronine, which recover after refeeding.
  • Fasting leads to extensive remodeling of plasma, IgG, and IgA N-glycosylation profiles, reflecting dynamic immune and metabolic adaptation, while most lipid fractions, liver enzymes, and electrolytes show only minor, reversible fluctuations.
  • Insulin and glucose levels decline during fasting and increase post-refeeding, though these changes are not statistically significant in this small pilot study, suggesting the protocol is feasible and well-tolerated, but findings require confirmation with larger studies.