Cytochrome P450 2E1 deficiency alters hepatic mitochondria and impairs liver organoid formation - PubMed
3 hours ago
- #CYP2E1 deficiency
- #Liver mitochondria
- #Sex differences
- Cytochrome P450 2E1 (CYP2E1) deficiency affects liver mitochondria and organoid formation differently in female and male mice.
- Female Cyp2e1 knockout (KO) mice show significant transcriptional changes in liver, with enriched mitochondrial pathways compared to wild-type females.
- Female KO livers exhibit impaired mitochondrial function and reduced mtDNA copy number, effects that are less pronounced in males.
- Cyp2e1 KO liver organoids have impaired growth, with male KO organoids growing slower and most female KO cells failing to form organoids.
- KO livers display biliary and hepatocellular abnormalities, more severe and prevalent in females, cautioning against broad CYP2E1 suppression therapies.