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Single-cell and spatial transcriptomics identify SPP1 + trophoblast necroptosis as a potential contributor to late-onset preeclampsia via SPP1-CD44-mediated interaction with macrophages - PubMed

3 hours ago
  • #Late-onset preeclampsia
  • #Spatial transcriptomics
  • #Necroptosis
  • The study used single-cell and spatial transcriptomics to analyze placental tissues from late-onset preeclampsia (LOPE) and healthy pregnancies.
  • It identified a distinct SPP1+ extravillous trophoblast (EVT) subtype linked to necroptosis, an inflammatory cell death pathway, as a key feature in LOPE.
  • Spatial analysis showed SPP1+ EVTs are localized near macrophages, with the SPP1-CD44 signaling axis mediating their interaction.
  • In a mouse model, Mlkl knockout reduced preeclampsia-like symptoms and SPP1 expression, supporting the role of necroptosis in LOPE.
  • The findings suggest necroptosis as a therapeutic target and SPP1 as a potential biomarker for LOPE pathogenesis.