Temporal control of Ninj1 activation determines cell-to-cell heterogeneity in IL-33 release - PubMed
2 hours ago
- #Ninj1
- #Cell Death
- #IL-33
- IL-33 release is monitored via IL-33-mCherry mouse cell lines at single cell resolution.
- Necroptosis leads to simultaneous SYTOX uptake and IL-33 release, indicating immediate membrane rupture by Mlkl.
- Apoptosis and pyroptosis show delayed IL-33 release in some cells, while others release it simultaneously with SYTOX uptake.
- Ninj1 knockout markedly reduces IL-33 release, while Gasdermins loss has only a modest effect, positioning them upstream.
- Variable timing of Ninj1 activation contributes to cell-to-cell heterogeneity in IL-33 release.