1,700 new protein found in the "dark proteome" of previously overlooked DNA
8 hours ago
- #proteomics
- #microproteins
- #ncORFs
- Approximately 25% of 7,264 non-canonical open reading frames (ncORFs) produce detectable peptides in a large-scale proteomics analysis of 95,520 experiments.
- The TransCODE Consortium develops an annotation framework for ncORF-encoded microproteins, introducing 'peptideins' as microproteins with indeterminate functional potential.
- An evolutionary analysis using ORF relative branch length (ORBL) shows that evolutionary constraint is common and linked to the observation of ncORF-derived peptides.
- A specific peptidein from the OLMALINC long non-coding RNA exhibits a pan-essential cellular phenotype, demonstrating biological relevance.
- Public tools from GENCODE and PeptideAtlas are generated to support research on understudied components of the human proteome.