Models for the architecture of the human inner kinetochore on centromeric α-satellite CENP-A nucleosome arrays - PubMed
2 hours ago
- #chromatin
- #kinetochore
- #cryo-EM
- Study presents cryo-EM structures of CCAN bound to free DNA and α-satellite repeats with CENP-A nucleosomes.
- CCAN engages 70 bp of DNA, including upstream α-satellite repeat, interacting with CENP-TWSX histone-fold domains.
- On dimeric α-satellite repeats, CCAN requires unwrapping DNA from CENP-TWSX and upstream nucleosome to fit on linker DNA.
- Results inform models for inner kinetochore architecture on centromeric α-satellite chromatin arrays.