Wumei Wan restores the colonic mucus barrier by modulating TGF-β/Smad signaling to promote goblet cell differentiation in ulcerative colitis - PubMed
2 days ago
- #TGF-β signaling
- #Goblet cell
- #Mucus barrier
- Wumei Wan (WMW), a traditional Chinese medicine formula, effectively treats ulcerative colitis by restoring the colonic mucus barrier.
- The study uses a DSS-colitis model and finds that WMW up-regulates goblet-cell proteins, expands mature and progenitor goblet cells, and accelerates epithelial progenitor differentiation toward goblet cells.
- WMW inhibits TGF-β-Smad2/3 signaling, which activates goblet cell differentiation, offering a therapeutic strategy distinct from simple anti-inflammatory approaches.
- Cross-scale validation includes quantitative proteomics, single-cell RNA sequencing, colonic organoids, Western blot, immunofluorescence, UHPLC-HRMS profiling, and molecular docking.