Single-cell RNA sequencing of terminal ileal biopsies identifies signatures of Crohn's disease pathogenesis - PubMed
2 hours ago
- #single-cell RNA sequencing
- #inflammation
- #Crohn's disease
- A large single-cell RNA sequencing dataset called IBDverse was created from terminal ileal biopsies of 111 Crohn's disease patients and 232 healthy controls.
- Epithelial changes were found, including interferon-driven upregulation of MHC class I molecules that persisted in progenitor cells even after inflammation resolved.
- ITGA4+ macrophages were identified as key inflammatory drivers, showing enriched JAK-STAT signaling and expression of cytokines like IL-6, IL-12, and IL-23.
- Heritability analysis linked inflammatory monocytes and macrophages to Crohn's disease susceptibility, implicating both resident and recruited immune cells in pathogenesis.
- The study provides a comprehensive cellular and molecular framework for Crohn's disease, offering insights into disease mechanisms and potential therapeutic targets.