Single-molecule tracking of RNA-DNA hybrid removal enzymes important for lagging-strand replication - PubMed
2 hours ago
- #DNA replication
- #single-molecule tracking
- #genome stability
- RNA-DNA hybrids (RDHs) are essential primers for DNA polymerase recruitment during replication initiation and Okazaki fragment synthesis.
- Persistent RDHs threaten genome stability by increasing mutation susceptibility, impairing replication forks, and causing DNA double-stranded breaks.
- This study tracks three RDH removal enzymes (Pol I, FenA, RNase HIII) in Bacillus subtilis using single-molecule imaging.
- All three enzymes are highly mobile, with residence times at substrates below temporal resolution.
- Pol I activity is modulated by replisome interaction, while FenA and RNase HIII are regulated by nucleoid access.