Structural basis for RISC assembly of human Argonaute2 - PubMed
3 hours ago
- #RISC assembly
- #cryo-EM
- #Argonaute
- The study presents cryo-EM structures of human AGO2 bound to an siRNA duplex, revealing intermediate states in RISC assembly.
- AGO2 uses its MID-PIWI lobe to secure one end of the duplex while α-helix 14 assesses duplex stability.
- The N domain, L1 hairpin, Stalk, and PAZ domain interact with the duplex, peeling the passenger strand from its 5' end.
- AGO2 fully ejects the passenger strand through target-assisted passenger ejection (TAPE), even with partially complementary target RNAs.
- The research shows that mRNAs are bifunctional, acting as both targets for RISCs and assisting in RISC assembly.