Transcriptomic profiling of the embryonic C. elegans intestine with single-cell resolution - PubMed
3 hours ago
- #single-cell RNA-seq
- #C. elegans
- #intestinal development
- Single-cell RNA sequencing of embryonic C. elegans intestinal cells identified 13 distinct clusters, indicating cellular diversity.
- Clusters were assigned to early, mid, and late developmental stages, with early stages expressing growth and cell division genes, and later stages expressing immune and metabolism genes.
- Gene expression varied along the anterior-posterior axis, with anterior-localized genes having a stronger impact on intestinal function when perturbed.
- The study combines transcriptomic data with smiFISH microscopy to map cluster identities to specific anatomical regions of the intestine.
- Findings highlight intrinsic heterogeneity in the 20-cell embryonic intestine, offering insights into specialization and future research on dietary and environmental responses.