Evolutionarily conserved and divergent mechanisms of dual Ca2+ sensors in synaptic vesicle exocytosis - PubMed
3 hours ago
- #Ca2+ sensor
- #neurotransmitter release
- #synaptic exocytosis
- The study explores dual Ca2+ sensor systems (SNT-1 and SNT-3) in C. elegans neuromuscular junctions, analogous to mammalian Syt1/Syt7.
- AlphaFold 3 modeling reveals a conserved C2B-SNARE interface similar to the canonical Syt1-SNARE interaction, with conserved binding residues.
- Electrophysiological analysis shows both SNT-1 and SNT-3 require C2B-SNARE interactions and polybasic motifs for fast and slow neurotransmitter release.
- SNT-1 and SNT-3 exhibit differential dependencies on specific C2B-SNARE interface regions and polybasic motifs, indicating distinct mechanisms for fast vs. slow release.
- SNT-1 mediates spontaneous neurotransmitter release via multiple pathways, including primary C2B-SNARE and additional SNARE-binding interactions.