Interplay between cohesin and TORC1 links chromosome segregation and gene expression to environmental changes - PubMed
3 hours ago
- #Environmental Adaptation
- #Chromosome Cohesion
- #TORC1 Pathway
- Cohesin is essential for chromosome structure and function, and defects in its loader Mis4 cause segregation issues and dysregulated gene expression in fission yeast.
- A suppressor screen of mis4 identified TORC1 mutants, linking the TORC1 pathway to cohesin's roles in chromosome segregation and gene expression regulation.
- Reduced TORC1 activity restored cohesin binding to chromosomes and improved mitotic segregation, even in wild-type cells, suggesting environmental cues impact chromosome stability via TORC1.
- TORC1 co-purified with cohesin, and lower TORC1 activity led to decreased phosphorylation of Mis4 and cohesin, with non-phosphorylated cohesin mutants mimicking TORC1 downregulation effects.
- The TORC1-Mis4 interaction also regulates stress-responsive genes, revealing a link that may aid adaptation to environmental changes, potentially connecting to lifespan effects of TORC1 inhibition.