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CircRNA based bi-antigen vaccines against mpox virus induce potent and durable cross-protection in mice - PubMed

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  • #immunity
  • #circRNA vaccine
  • #mpox virus
  • CircRNA bi-antigen vaccines cirEV and cirMV were developed against MPXV using lipid nanoparticle encapsulation, encoding EEV (A35R and B6R) or IMV (A29L and M1R) antigens.
  • The vaccines induced robust antibody and cellular immune responses in mice, providing dose-dependent protection against lethal VACV challenge and durable cross-protection for up to 260 days post-immunization.
  • Cellular immune responses were critical for immune protection and virus clearance, highlighting circRNA vaccines as a promising multivalent platform with insights into protective mechanisms in an orthopoxvirus model.