Region-specific transcriptional signatures of brain aging in the absence of neuropathology at the single-cell level - PubMed
3 days ago
- #brain aging
- #single-cell transcriptomics
- #region-specific signatures
- The study uses single-nucleus RNA sequencing (snRNA-seq) to analyze age-related gene expression changes in the human brain, focusing on individuals without neuropathology.
- Samples were collected from six young (20-30 years) and seven aged (60-85 years) individuals across four brain regions: entorhinal cortex, middle temporal gyrus, subventricular zone, and putamen.
- Over 150,000 nuclei were captured, representing 10 broad cell-types, with more than 8,000 age-associated genes identified through region- and cell-type-specific differential expression analyses.
- Most age-associated gene changes were found to be region-specific within a given cell-type, highlighting distinct transcriptional aging profiles across brain regions and cell types.
- Functional enrichment analyses linked these gene sets to hallmarks of aging, including proteostasis, cytokine interactions, vesicular trafficking, metabolism, inflammation, metal ion homeostasis, and cellular senescence.