A cytosolic IF1 reporter enables real-time visualization of severe mitochondrial membrane damage - PubMed
3 hours ago
- #IF1 Reporter
- #Lysosomal Clearance
- #Mitochondrial Damage
- The article introduces MAI-1, a cytosolic IF1 reporter, as the first genetically encoded tool to visualize severe mitochondrial membrane damage in real-time.
- MAI-1 is derived from Caenorhabditis elegans and translocates irreversibly to damaged mitochondria within milliseconds, with conserved behavior across species.
- Recruitment of MAI-1 to damaged mitochondria depends on an intact ATP synthase complex.
- Using MAI-1 as a sensor, the study reveals that severely damaged mitochondria are cleared via an LGG-1-mediated lysosomal pathway, independent of PINK1/PARKIN.
- The findings provide a new tool for monitoring mitochondrial integrity and uncover a surveillance mechanism for structural damage control.