Influence of protein aggregates, extracellular vesicles, and lipoprotein fusion on ionizable lipid nanoparticles protein corona analysis - PubMed
3 hours ago
- #protein corona
- #lipid nanoparticles
- #proteomics
- Ionizable lipid nanoparticles (LNPs) are key for nucleic acid therapeutics since 2018, but extrahepatic delivery is hindered by rapid hepatic uptake from apolipoprotein adsorption.
- Analyzing the LNP protein corona is crucial for organ-specific targeting, but soft materials pose analytical challenges, with contaminants like extracellular vesicles and lipoproteins often obscuring true corona composition.
- Refined proteomic strategies are needed to differentiate genuine corona proteins from impurities, as the LNP protein corona likely differs significantly from rigid shells on inorganic nanoparticles.