TurboID-Based Proximity Labeling Map Reveals the Protein Interaction Network and Anti-Viral Mechanism Against Influenza A H1N1 - PubMed
3 hours ago
- #TurboID
- #Protein Interaction
- #Influenza A H1N1
- Used TurboID proximity labeling to map interactions between 10 H1N1 viral proteins and host proteins in human alveolar epithelial cells.
- Identified HA and PA proteins binding to MAVS, suppressing IFN-β promoter activity and impairing TBK1/STAT signaling.
- Found HA exploits ITGA2 as a novel entry cofactor; silencing ITGA2 reduced viral titers.
- TRIM56 restricts H1N1 replication by binding NP and inducing K48-linked ubiquitination for proteasomal degradation.
- Highlighted MAVS, ITGA2, and TRIM56 as key targets for combination therapies against influenza.