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Programmable antibody-based chimeric entry receptors for sarbecoviruses - PubMed

4 hours ago
  • #ABCERs
  • #vaccine cross-protection
  • #sarbecoviruses
  • Research introduces antibody-based chimeric entry receptors (ABCERs) for sarbecoviruses, replacing hACE2's extracellular domain with scFvs from neutralizing antibodies.
  • ABCERs convert antibody specificity into a programmable entry module, enabling cathepsin L-dependent endocytic fusion for diverse sarbecovirus infection.
  • E7 scFv demonstrates exceptional breadth, recognizing conserved epitopes across all sarbecovirus clades, including those independent of hACE2.
  • Pfizer-BioNTech mRNA-vaccinated sera potently block E7 binding to SARS-CoV-2 but show limited cross-inhibition for hACE2-independent sarbecoviruses.
  • E7-based ABCERs serve as a synthetic receptor platform for isolating, studying, and surveying sarbecoviruses beyond hACE2 dependence.