Urinary fluorogenic reporters for noninvasive detection and staging of kidney fibrosis - PubMed
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- #Fluorogenic Reporters
- #Noninvasive Diagnosis
- #Kidney Fibrosis
- Reporters called fibrogenesis sensing reporters (FSRs) were developed for noninvasive detection of kidney fibrosis.
- FSRs target two up-regulated biomarkers in fibrotic kidneys: lysyl oxidase-derived allysine and transglutaminase 2 (TG2), enhancing diagnostic accuracy.
- These reporters are initially nonfluorescent and clear renally, becoming fluorescent upon TG2 cleavage, enabling detection via imaging or urine assays.
- FSR-based urinalysis distinguished kidney fibrosis from nonfibrotic acute injury and extrarenal fibrosis, offering specificity not found in standard tests.
- In a clinical trial with 35 patients, FSR urinalysis showed 84% sensitivity and 94% specificity for detecting chronic kidney disease and discriminated mild from severe fibrosis.
- Traditional metrics like estimated glomerular filtration rate and serum creatinine failed to classify fibrosis severity, highlighting FSR's potential for improved patient management.