An in vivo parallelized reporter assay to uncover tissue-specific splicing regulatory sequences in a multicellular animal - PubMed
3 hours ago
- #splicing regulation
- #in vivo assay
- #Caenorhabditis elegans
- Massively parallel reporter assays (PRA) were implemented in C. elegans neurons and muscle cells to screen intronic enhancer and silencer motifs.
- The study identified nearly 200 sequences regulating splicing, including motifs recognized by known RNA-binding proteins and unmapped motifs.
- PRA-derived motifs found in native introns flanking tissue-biased alternative exons are conserved across nematodes, supporting functional relevance.
- Individual intronic regions often contain diverse motif combinations, suggesting complex RNA-binding protein engagement.
- Targeted mutagenesis of intronic enhancers identified key cis-regulatory determinants for neuronal microexon splicing.