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PCK2-Mediated PQBP1 Lactylation Promotes Asthmatic Inflammation through PRMT5 Inhibition - PubMed

5 hours ago
  • #epigenetics
  • #asthma
  • #metabolism
  • Asthmatic airways show lactate-driven glutaminolysis leading to lactate and succinate accumulation.
  • PCK2 succinylation at K100 enhances its stability by antagonizing ubiquitination, creating a lactate-generating feedback loop.
  • Accumulated lactate triggers PQBP1 lactylation at K223, which inhibits the PRMT5/WDR77 complex.
  • This inhibition erases H4R3me2s repressive marks from proinflammatory gene promoters, causing transcriptional derepression.
  • Airway epithelium-specific Pqbp1 knockout reduces inflammation, goblet cell hyperplasia, and Th2 responses.
  • Pck2-shRNA or oxamate treatment ameliorates asthmatic pathology.
  • The study identifies a PCK2-lactate-PQBP1-PRMT5 axis linking metabolic reprogramming to epigenetic dysregulation in asthma.