A nanopore-based HIV-1 reference epitranscriptome - PubMed
7 hours ago
- #nanopore
- #epitranscriptome
- #HIV-1
- Post-transcriptional RNA modifications (epitranscriptome) influence RNA metabolism, gene regulation, and disease.
- A reference epitranscriptome for HIV-1 was established using nanopore sequencing technology.
- Modifications mapped include N6-methyladenosine, 5-methylcytidine, pseudouridine, inosine, and 2'-O-methyl.
- Synthetic RNA fragments corrected modification miscalling due to sequence context.
- Modifications were consistent under antiretroviral therapy, in primary CD4+ T cells, and HIV-1 virions.
- Spliced transcript-dependent modification levels were observed.
- Conservation of m6A modifications in circulating HIV strains from patients was noted.
- The study provides a benchmark for HIV-1 epitranscriptomics and a roadmap for other pathogens.