Development of Intervertebral Disc Organoids through Directed Differentiation of Mesenchymal Stem Cells and Hierarchical 3D Printing - PubMed
5 hours ago
- #IVD repair
- #3D printing
- #organoids
- Development of intervertebral disc (IVD) organoids from human mesenchymal stem cells (hMSCs) using differentiation media with folic acid (FA) and connective tissue growth factor.
- FA promotes nucleus pulposus (NP) cell generation via modulation of PI3K-AKT and TGF-β pathways.
- Hierarchical 3D printing used to create biomimetic IVD scaffolds with host-guest and silk fibroin hydrogels for NP and AF organoids.
- Manual assembly of IVD scaffolds and organoids into complex IVD organoids (IVDOs) mimicking natural IVD structure.
- Implantation of IVDOs into goat lumbar spine post-discectomy shows anisotropic IVD reconstruction.
- Potential for IVD organoids in research on disc degenerative diseases and therapeutic applications.