High-resolution imaging method details nerves across a mouse’s body
10 months ago
- #connectomics
- #imaging
- #neuroscience
- A new imaging method maps mouse nerves from brain and spinal cord to the body at micrometre-scale resolution.
- The method involves treating the mouse with chemicals to make tissues transparent and labeling nerves with fluorescent markers.
- A device combines a slicing tool and a microscope to take 3D images, advancing the body 400 micrometres at a time.
- The process is faster than other techniques, reducing risks of mechanical failure, signal loss, or sample degradation.
- Researchers tested the method on 16 adult mice using three distinct nerve-marking techniques.