An ultraconserved pseudo 5' splice site fine-tunes development by regulating alternative splicing within TOR-related pathways - PubMed
14 hours ago
- #ultraconserved RNA
- #alternative splicing
- #TOR pathway
- Ultraconserved pseudo 5' splice sites (UCP-5'SSs) regulate alternative splicing in TOR-related pathways.
- Deletion of a 9-nt UCP-5'SS in Drosophila leads to enlarged ovaries and increased fecundity.
- UCP-5'SS acts as a silencer for alternative splicing by interacting with U1 snRNP-core proteins.
- AS changes are observed in dTOR and Insulin-like pathway mutants, mitigated by UCP-5'SS loss.
- Human cells treated with mTOR/Insulin pathway inhibitors show AS changes and increased U1-70K translation.
- The study reveals a conserved regulatory network where intronic RNA elements sense TOR-related pathways.